There are several elements in chemistry which were given quite much attention because of the properties they posses which were suitable for many kind of experiment. One of which is the dibenzocyclooctyne or DBCO because this element is known to be thermally stable in terms of high specific reactivity towards the azide group. This is done through a strain promoted of click reactions without any presence of catalyst on the process. This ligation reaction is quite fast and its result shows in a quantitative yield of some stable triazoles. This is proven so long as its within the physiological temperatures as well as some pH ranges.
Its unique sets of reaction could be mesmerizing and no wonder scientists has been so keen and close to studying these groups. This has helped a lot in the click chemistry field of study because of its input that were not found on other compounds. Its various reaction was made without any involvement of copper or catalyst in it.
Usually, it ranges from the protein visualization of its expression to the tracking of its localization. It as well could measure the activity of protein along with its turnover to the nucleic acid immobilization as well as tracking. Last of its range capability is the lipid labeling in the living cells of live animals.
Such groups actually holds several remarkable feature and benefits that makes its presence useful to science. Few of which is on its biocompatibility which is said to have a no cytotoxic copper and catalyst required at all. This allows a nice to in vivo applications.
Most often than not, these groups were used to visualize the expression of proteins. They would normally do it through tracking its localization. That allows the measuring of activity found on protein available and possible.
They were also to try it along with turning over of nucleic acid into immobilization as tracking occurs. Because of that, they have noticed something strange on its capability. They were able to see a strong chance of lipid labeling on most living cells located on animals which were alive.
The conjugation chemistry is merely based on reaction of the dibenzocyclooctyne groups with the azide linker which then can form the stable triazole. It is quite rapid when done in a room temperature. It would normally nor require the catotoxic to even react that way.
Furthermore, they have been proving their unique attributes as its complementary means were different than the rest. Usually, they have a minimal reaction especially of aqueous media. Mild compatibility was only found in them.
True enough, the dibenzocyclooctyne azide conjugation is said to be complementary. It makes their reaction merely unique towards each other and their series or reaction would then require three step method to be entirely successful. These steps are activation of first biomolecule with the dibenzocyclooctyne followed by another activation with the second biomolecule along with the azide. The two activated molecules is mixed to form a conjugate.
Its unique sets of reaction could be mesmerizing and no wonder scientists has been so keen and close to studying these groups. This has helped a lot in the click chemistry field of study because of its input that were not found on other compounds. Its various reaction was made without any involvement of copper or catalyst in it.
Usually, it ranges from the protein visualization of its expression to the tracking of its localization. It as well could measure the activity of protein along with its turnover to the nucleic acid immobilization as well as tracking. Last of its range capability is the lipid labeling in the living cells of live animals.
Such groups actually holds several remarkable feature and benefits that makes its presence useful to science. Few of which is on its biocompatibility which is said to have a no cytotoxic copper and catalyst required at all. This allows a nice to in vivo applications.
Most often than not, these groups were used to visualize the expression of proteins. They would normally do it through tracking its localization. That allows the measuring of activity found on protein available and possible.
They were also to try it along with turning over of nucleic acid into immobilization as tracking occurs. Because of that, they have noticed something strange on its capability. They were able to see a strong chance of lipid labeling on most living cells located on animals which were alive.
The conjugation chemistry is merely based on reaction of the dibenzocyclooctyne groups with the azide linker which then can form the stable triazole. It is quite rapid when done in a room temperature. It would normally nor require the catotoxic to even react that way.
Furthermore, they have been proving their unique attributes as its complementary means were different than the rest. Usually, they have a minimal reaction especially of aqueous media. Mild compatibility was only found in them.
True enough, the dibenzocyclooctyne azide conjugation is said to be complementary. It makes their reaction merely unique towards each other and their series or reaction would then require three step method to be entirely successful. These steps are activation of first biomolecule with the dibenzocyclooctyne followed by another activation with the second biomolecule along with the azide. The two activated molecules is mixed to form a conjugate.
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